Analysis by Isopycnic centrifugation of isolated nucleoids of Escherichia coli
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Analysis by isopycnic centrifugation of isolated nucleoids of Escherichia coli.
The isolated, formaldehyde-fixed nucleoid of E. coli has been analyzed by isopycnic centrifugation in CsCl density gradients. The membrane-free nucleoid bands at a density of 1.69 +/- 0.02 g/cm3. The membrane-associated nucleoid bands at a density of 1.46 +/- 0.02 g/cm3. Both species sediment to equilibrium as nearly monodisperse bands in CsCl, suggesting that the nucleoid components of DNA, RN...
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Fritsch, A. (1973) Anal. Biochem. 35,57-71 Griffith, 0. M. (1978) Anal. Biochem. 9 0 , 4 3 5 4 4 3 Hames, B. D. & Rickwood, D. (1978) in Centr&gation-A Practical Approach (Rickwood, D., ed.), pp. 69-80, Information Retrieval. London Ridge, D. (1978) in Centrifugal Separations in Molecular and Cell Biology (Birnie, G. D. & Rickwood, D., eds.), pp. 33-61. Butterworths, London Young, B. D. (1978) ...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1975
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/2.9.1559